Comprehensive, high resolution analysis of cis-acting regulatory networks at the alpha globin locus (HTS)

ABSTRACT: We have combined the 4C chromosome conformation capture protocol with high throughput, genome-wide sequence analysis to characterise in full a cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000kb), the 4C approach provides a comprehensive, high resolution analysis of a specific locus with the aim of defining, in detail, all cis- regulatory elements controlling a single gene or gene cluster. Using the human M-NM-1-globin locus as a model we detected all known local and long-range interactions with this gene cluster. In addition we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the M-NM-1-globin cluster. Using paired-end sequencing it was possible for the first time to identify inte

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Gibbons 

PROVIDER: E-GEOD-42382 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress