Comparison of DNA methylation profilings between hESCs and hiPSCs

ABSTRACT: It remains controversial whether human induced pluripotent stem cells (hiPSCs) are distinct from human embryonic stem cells (hESCs) in their molecular signatures and differentiation properties. We examined the gene expression and DNA methylation of 49 hiPSC and 10 hESC lines and identified no molecular signatures that distinguished hiPSCs from hESCs. Comparisons of the in vitro directed neural differentiation of 40 hiPSC and four hESC lines showed that most hiPSC clones were comparable to hESCs. However, in seven hiPSC clones, significant amount of undifferentiated cells persisted even after neural differentiation and resulted in teratoma formation when transplantated into mouse brains. These differentiation-defective hiPSC clones were marked by higher expression of several genes, includin

ORGANISM(S): Homo sapiens

SUBMITTER: Shinya Yamanaka 

PROVIDER: E-GEOD-42448 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress