Defining a molecular roadmap of cellular reprogramming into iPS cells [miRNA profiling]

ABSTRACT: Factor induced reprogramming is a slow and inefficient process with only rare cells progressing towards induced pluripotent stem cells (iPSCs). Owing to these restraints, mechanistic studies have been limited to analyses of heterogeneous bulk populations undergoing reprogramming and partially reprogrammed cell lines. Here, by combining surface markers (Thy1, SSEA1) and an Oct4-GFP fluorescent reporter allele, we analyzed defined intermediate cell populations poised to becoming iPSCs at the transcriptional and epigenetic levels using genome-wide and single cell technologies. We found that factor-induced reprogramming elicits two discernible transcriptional waves that are characterized by the initial extinction of the somatic gene expression program and the concomitant acquisition of an ESC-

ORGANISM(S): Mus musculus

SUBMITTER: Ben Wittner 

PROVIDER: E-GEOD-42475 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress