Transcription profiling of mouse thymocytes from ild type or RORg-/- animals

ABSTRACT: Microarray analysis was performed in order to identify changes in gene expression in thymocytes isolated from RORg-/- mice in comparison to those of wild type mice. Experiment Overall Design: Microarray analyses were carried out by the NIEHS Microarray Group (NMG). Gene expression analysis was conducted using Agilent Mouse arrays (Agilent Technologies, Palo Alto, CA) representing about 20,000 genes. For each condition, equal amounts of total RNA from thymocytes of three individual wild type or RORg-/- mice were pooled and amplified. Total RNA was amplified using the Agilent Low RNA Input Fluorescent Linear Amplification Kit protocol. Starting with 1 mg of total RNA, Cy3- or Cy5-labeled cRNA was synthesized according to manufacturer’s protocol. For each comparison, 750 ng of each Cy3- a

ORGANISM(S): Mus musculus

SUBMITTER: NIEHS Microarray Group 

PROVIDER: E-GEOD-4248 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress