High-throughput sequencing of small RNA transcriptome reveals salt stressed regulated microRNAs in sugarcane

ABSTRACT: Salt stress is a primary cause of crop losses worldwide, and it has been the subject of intense investigation to unravel the complex mechanisms responsible for salinity tolerance. MicroRNA is implicated in many developmental processes and in responses to various abiotic stresses, playing pivotal roles in plant adaptation. Deep sequencing technology was chosen to determine the small RNA transcriptome of Saccharum sp cultivars grown on saline conditions. We constructed four small RNAs libraries prepared from plants grown on hydroponic culture submitted to 170mM NaCl and harvested after 1h, 6hs and 24hs. Each library was sequenced individually and together generated more than 50 million short reads. Ninety-eight conserved miRNAs and 33 miRNAs* were identified by bioinformatics. Several of t

ORGANISM(S): Saccharum hybrid cultivar SP70-1143

SUBMITTER: Paulo Ferreira 

PROVIDER: E-GEOD-42484 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress