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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Induced pluripotent stem cell-derived neurons from a sporadic Alzheimer disease donor as a model for investigating disease mechanisms

ABSTRACT: Gene expression analysis of control fibroblasts (NFH2), one AD-derived fibroblasts (NFH-46), NFH2-derived control-iPS cells (OiPS3, OiPS6), NFH46-derived AD-iPS cells (iPS5 and iPS 26B), hESCs (H1 and H9). Total mRNA obtained from AD fibroblasts (NFH-46) and control fibroblasts (NFH-2), from pluripotent stem cells AD-iPS cells (iPS5 and iPS 26B), control-iPS cells (OiPS3, OiPS6), and hESCs (H1 and H9).

ORGANISM(S): Homo sapiens

SUBMITTER: Alessandro Prigione

PROVIDER: E-GEOD-42492 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Induced pluripotent stem cell-derived neuronal cells from a sporadic Alzheimer's disease donor as a model for investigating AD-associated gene regulatory networks.

Hossini Amir M AM Megges Matthias M Prigione Alessandro A Lichtner Bjoern B Toliat Mohammad R MR Wruck Wasco W Schröter Friederike F Nuernberg Peter P Kroll Hartmut H Makrantonaki Eugenia E Zouboulis Christos C CC Adjaye James J

BMC genomics 20150214

<h4>Background</h4>Alzheimer's disease (AD) is a complex, irreversible neurodegenerative disorder. At present there are neither reliable markers to diagnose AD at an early stage nor therapy. To investigate underlying disease mechanisms, induced pluripotent stem cells (iPSCs) allow the generation of patient-derived neuronal cells in a dish.<h4>Results</h4>In this study, employing iPS technology, we derived and characterized iPSCs from dermal fibroblasts of an 82-year-old female patient affected b ...[more]

PMID: 25765079

Similar Datasets

Induced pluripotent stem cell-derived neurons from a sporadic Alzheimer disease donor as a model for investigating disease mechanisms

Project description:Gene expression analysis of control fibroblasts (NFH2), one AD-derived fibroblasts (NFH-46), NFH2-derived control-iPS cells (OiPS3, OiPS6), NFH46-derived AD-iPS cells (iPS5 and iPS 26B), hESCs (H1 and H9).

2015-04-21 | GSE42492 | GEO

Human Induced Pluripotent Stem Cells Harbor Homoplasmic and Heteroplasmic Mitochondrial DNA Mutations While Maintaining human embryonic stem cells-like Metabolic Reprogramming

Project description:Gene expression analyis of two hESCs, two human neonatal fibroblasts, and four human iPSCs generated with retroviral transduction using the OSKM cocktail. Total mRNA obtained from two fibroblasts (BJ and HFF1), two hESCs (H1 and H9), two BJ-derived iPSCs (iB4 and iB5), and two HFF1-derived iPSCs (iPS2 and iPS4).

2011-07-08 | E-GEOD-26575 | biostudies-arrayexpress

HIF1alpha drives early induction of pluripotency through reprogramming of glycolytic metabolism

Project description:Gene expression analyis of two neonatal fibroblasts (BJ and HFF1), one adult dermal fibroblasts (NFH2), two BJ-derived human iPSCs (iB4 and iB5), two HFF1-derived iPSCs (iPS 2 and iPS4), four NFH2-derived iPSCs (OiPS3, OiPS6, OiPS8, OiPS16), one amniotic fluid cells and three derived iPSCs (lines 4, 5, 6, 10, and 41), two human ES cells (H1 and H9), neonatal fibroblasts transduced with the four retroviral factors (OKSM) after 24h, 48h, and 72h, neonatal fibroblasts treated with EDHB for 24h, 48h, and 72h, neonatal fibroblasts transduced with four factors and treated with EDHB for 24h, 48h, and 72h, neonatal fibroblasts knocked down for HIF1A (HIF1-KD) and for a scrambled sequence (SCR-KD) Total mRNA obtained from somatic cells (fibroblasts and amniotic fluid cells), pluripotent stem cells (iPSCs and hESCs), and somatic cells exposed to HIF1A activation or HIF1A suppsression in addition to the standard retroviral-based reprogramming.

2013-10-01 | E-GEOD-37709 | biostudies-arrayexpress

Molecular insights into induced pluripotency mediated by the OCT4, SOX2, KLF and c-MYC gene regulatory network

Project description:Human somatic fibroblasts can be reprogrammed to induced pluripotent stem (iPS) cells by exogenic expression of the Yamanaka factors (OCT4, SOX2, KLF4 and MYC) after about 1 month. To gain some insight into the early processes operative in fibroblast reprogramming, we profiled genome-wide transcription levels using Illumina microarrays in the starting donor cells-human foreskin fibroblast (HFF1) cells and at three time points after OSKM transduction (24 h, 48 h, 72 h), as well as two iPS cell lines (iPS2, iPS4) and hES cell lines (H1, H9). We show that within the context of the viral transduction reprogramming protocol, the donor cell response to viral transfection perturbs redox homeostasis, which induces oxidative damage on the donor cells' protein and DNA. This leads to activation of p53, senescence, and apoptosis, greatly reducing the efficiency of reprogramming. Total RNA obtained from HFF1 (human foreskin fibroblast) cells, OSKM-transduced HFF1 cells after 24h, 48h, 72h, undifferentiated hESCs, iPSCs.

2011-10-24 | E-GEOD-28688 | biostudies-arrayexpress

Nucleosome profiling in human embryonic stem cells

Project description:Paired-end sequencing of MNase digested H1 and H9 hESCs. Paired-end sequencing of MNase digested H1 and H9 hESCs

2015-05-04 | E-GEOD-49140 | biostudies-arrayexpress

Nucleosome profiling in human embryonic stem cells

Project description:Paired-end sequencing of MNase digested H1 and H9 hESCs.

2015-05-04 | GSE49140 | GEO

Reprogramming human fat cells into iPS cells

Project description:This study determined whether human adipose stem cells (hASCs) could be reprogrammed into induced pluripotent stem (iPS) cells using the 4 factors OCT4, SOX2, KLF4, and cMYC. We showed successfull reprogramming could be achieved in which fat/adipose cells were able to assume embryonic-like phenotypes after lentiviral transduction with the 4 factors. Further, we believe these fat cells are intrinsically better-suited for reprogramming compared to fibroblasts, and result in higher yields of iPS cells. Microarray study was performed in duplicates (hASCs, iPS cells derived from hASCs, and hESCs). Reference control was pooled RNA taken from H9 hESCs, embryoid bodies, and some differentiated cell types such as endothelial cells.

2009-02-26 | E-GEOD-14982 | biostudies-arrayexpress

Direct Reprogramming of Human Neural Stem Cells by OCT4

Project description:Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of the four transcription factors (OCT4, SOX2, c-MYC, KLF4). We previously reported that Oct4 alone is sufficient to directly reprogram adult mouse neural stem cells (NSCs) to iPS cells. Here, we report the generation of one-factor (1F) human iPS from human NSCs (1F hNiPS) by ectopic expression of Oct4 alone. 1F hNiPS cells resemble human embryonic stem cells (hESCs) in global gene expression profiles, epigenetic status and pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human NSCs to pluripotency. 1F iPS cell generation will accelerate this field further towards understanding reprogramming and generating patient-specific pluripotent stem cells. For transcriptome profiling, 400 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 10h). Quality-checked cRNA samples were hybridized as biological or technical duplicates for 18 h onto HumanRef-8 v3 expression BeadChips (Illumina), washed, stained, and scanned following guidelines and using materials / instrumentation supplied / suggested by the manufacturer. Five sample types were analyzed, each one of them in duplicate. hNSC: human fetal neural stem cells (duplicates); 1F hNiPS: One factor (Oct4) human iPS cells from hNSCs, hand-picked cols (duplicates); 2F hNiPS: Two factors (Oct4,Klf4) human iPS cells from hNSCs, hand-picked cols (duplicates); H9 hESC: H9 human ESCs grown on low-density CF1 MEFs (duplicates); H1 hESC: H1 human ESCs grown on low-density CF1 MEFs (duplicates).

2010-05-20 | E-GEOD-15355 | biostudies-arrayexpress

HIF1alpha drives early induction of pluripotency through reprogramming of glycolytic metabolism

2013-10-01 | GSE37709 | GEO

Discovery of consensus gene signature and intermodular connectivity defining self-renewal of human embryonic stem cells

Project description:Comparison of gene expression signatures in undifferentiated hESCs against differentiated embryoid bodies to identify key signatures defining self-renewal of hESCs. Using H1 and H9 hESC lines, we performed gene expression microarray analysis (Affymetrix Human Genome U133 Plus 2.0 Array) and analyzed the interaction patterns of 54,614 genes using WGCNA in R programming

2014-01-18 | E-GEOD-54186 | biostudies-arrayexpress

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