Comparison of the transcriptome of the conditional afpig-a mutant and the wild-type

ABSTRACT: In order to gain a better understanding of the mechanism of increased cell death triggered by deficient GPI anchoring, we constructed a whole genome microarray using the Agilent eArray 5.0 program.The chip specification is 8M-CM-^W15K. The genome sequences were downloaded from the following site:http://www.ncbi.nlm.nih.gov/genome?Db=genome&Cmd=Search&Term=txid330879[orgn]. Each gene was represented by one 60-nt oligonucleotide probe, and 358 genes out of the 9630 total were replicated 14 times.Using this microarray,we got 3274 genes which were induced or reduced at least 1.5 fold in the afpig-a mutant comparing to the wild-type. Mycelia of the afpig-a mutant and the wild-type were harvested from strains grown in liquid CM at 37M-BM-0C for 36h with shaking at 200 rpm and ground by hand.Tota

ORGANISM(S): Aspergillus fumigatus

SUBMITTER: jianghong yan 

PROVIDER: E-GEOD-42499 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress