Pilot study to identify technical variation in microarray data of mouse lungs in the context of inter-animal variation

ABSTRACT: Prior to generation of microarray data for a Top-down Systems analysis of influenza A infection, we evaluated the degree and origin of technical variation in gene expression microarray data from mouse lungs and compared this to inter-animal variation. Technical variation in these microarray data in the context of inter-animal variation supported a choice of biological rather than technical replicates. A) One mouse (260) was infected with 0.6LD50 of the mouse pathogenic H1N1 strain PR8. Lung tissues were collected at 72h post infection and processed to yield whole lung RNA that was used for microarray analysis. The RNA was extracted in 3 individual batches (I, II, III), each batch of RNA was amplified in 2 separate batches (1,2), and the individual batches of amplified product were again hy

ORGANISM(S): Mus musculus

SUBMITTER: Marlene Brandes 

PROVIDER: E-GEOD-42521 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress