Transcription profiling by array of yeast single and double deletion mutants of gene-specific transcription factors

ABSTRACT: Contains gene expression profiles of yeast single and double deletion mutants of gene-specific transcription factors. Genetic interactions were studied by comparing gene expression changes of double mutants with gene expression changes in the respective single mutants. Pairs of gene-specific transcription factors were chosen based on previous evidence for epistasis, including synthetic genetic interactions as well as common DNA binding. Two channel microarrays were used. RNA isolated from a large amount of wt yeast from a single culture was used as a common reference. This common reference was used in one of the channels for each hybridization and used in the statistical analysis to obtain an average expression-profile for each deletion mutant relative to the wt. Two independent cultures w

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Patrick Kemmeren 

PROVIDER: E-GEOD-42536 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress