Decoupling chromatin and genetic effects through systematic analysis of gene position

ABSTRACT: Classic ‘position effect’ experiments repositioned genes to the telomere to demonstrate that the epigenetic landscape can dramatically alter gene expression. Here we show that systematic gene knockout collections provide an exceptional resource for interrogating position effects, not only at the telomere but at every single genetic locus. Because deleted genes are replaced by the same reporter gene, interrogation of this reporter provides a sensitive probe into many different chromatin environments while controlling for genetic context. Using this approach we find that, whereas replacement of yeast genes with the kanMX marker does not perturb the chromatin landscape, differences due to gene position account for more than 35% of marker gene activity. We observe chromatin influences differen

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Menzies Chen 

PROVIDER: E-GEOD-42554 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress