Quiescent Fibroblasts Exhibit High Metabolic Activity

ABSTRACT: Many cells in mammals exist in the state of quiescence, which is characterized by reversible exit from the cell cycle. Quiescent cells are widely reported to exhibit reduced size, nucleotide synthesis, and metabolic activity. Much lower glycolytic rates have been reported in quiescent compared with proliferating lymphocytes. In contrast, we show here that primary human fibroblasts continue to exhibit high metabolic rates when induced into quiescence via contact inhibition. By monitoring isotope labeling through metabolic pathways and quantitatively identifying fluxes from the data, we show that contact-inhibited fibroblasts utilize glucose in all branches of central carbon metabolism at rates similar to those of proliferating cells, with greater overflow flux from the pentose phosphate pat

ORGANISM(S): Homo sapiens

SUBMITTER: Eric Suh 

PROVIDER: E-GEOD-42612 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress