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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Structural systems biology evaluation of metabolic thermotolerance in Escherichia coli

ABSTRACT: Investigation of whole genome gene expression level in E. coli K-12 MG1655 in glucose M9 minimal media with/without heatshock A six chip study using total RNA recovered from E. coli K-12 MG1655 grown up to OD600nm 0.6 (mid-exponential phase) in M9 minimal media supplemented with 0.2% glucose with/without heatshock in 42oC. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes with 50-bp length that are spaced 25 bp apart across the E. coli genome (NimbleGen). Experiments were performed with three biological replicates.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Donghyuk Kim

PROVIDER: E-GEOD-42675 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Structural systems biology evaluation of metabolic thermotolerance in Escherichia coli.

Chang Roger L RL Andrews Kathleen K Kim Donghyuk D Li Zhanwen Z Godzik Adam A Palsson Bernhard O BO

Science (New York, N.Y.) 20130601 6137

Genome-scale network reconstruction has enabled predictive modeling of metabolism for many systems. Traditionally, protein structural information has not been represented in such reconstructions. Expansion of a genome-scale model of Escherichia coli metabolism by including experimental and predicted protein structures enabled the analysis of protein thermostability in a network context. This analysis allowed the prediction of protein activities that limit network function at superoptimal tempera ...[more]

PMID: 23744946

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