Identification and Correction of Amplification Protocol Bias in Microarray Studies

ABSTRACT: mRNA abundance microarrays are the most widely used high-throughput technology for functional genomics. But despite this extensive characterization, and despite their wide-spread adoption in discovery research, the uptake of microarrays as clinical diagnostic tools remains relatively limited due to sample degradation. Recently, the NuGEN WT-Ovation Pico kit was developed to resolve this problem. Here, we comprehensively evaluate its utility on two separate biological problems. First, we evaluate its effect on 12 malignant and 2 normal cervix samples. Second, we evaluate its effect on 2 normal kidney samples. In each case, we directly compare aliquots of the same RNA sample that are prepared by standard and low-input/low-RIN protocols to address three key questions. First, does the use of a

ORGANISM(S): Homo sapiens

SUBMITTER: Rui Yan 

PROVIDER: E-GEOD-42764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress