Transcription profiling of human PBMC from autoimmune ear disease (AIED) patients to assess IL1R2 and glucocorticoid response

ABSTRACT: Glucocorticoid responsive AIED patients demonstrate alternate splicing of IL1R2 in response to dexamethasone prior to treatment. Identification of IL1R2 as a potential target gene in AIED was determined by a microarray analysis of patients with end-stage AIED and controls with non-immunologic etiologies of hearing loss undergoing cochlear implantation. Experiment Overall Design: Patients with either end-stage AIED or controls with non-immunologic etiologies of hearing loss undergoing cochlear implantation were enrolled. All patients received pneumovax 2 weeks prior to surgery. At the time of surgery, blood was taken for PBMC isolation and autologous perilymph was harvested from a bloodless field. For each patient, 3 microarrays were performed: (1) unstimulated PBMC, (2) pneumovax stimu

ORGANISM(S): Homo sapiens

SUBMITTER: Andrea Vambutas 

PROVIDER: E-GEOD-4277 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress