Induced pluripotent stem cells can be generated in bulk culture

ABSTRACT: Induced pluripotent stem cells (iPSCs) are usually clonally derived. The selection of fully reprogrammed cells generally involves picking of individual colonies with morphology similar to embryonic stem cells (ESCs). However, successfully reprogrammed cells are highly proliferative and escape from cellular senescence - it is therefore conceivable that they outgrow non-pluripotent and partially reprogrammed cells during culture expansion without the need of clonal selection. In this study, we have reprogrammed human dermal fibroblasts (HDFs) with episomal plasmid vectors. Colony frequency and size was higher when using murine embryonic fibroblasts (MEFs) as stromal support instead of HDFs or human mesenchymal stromal cells (MSCs). We have then compared iPSCs which were either clonally deriv

ORGANISM(S): Homo sapiens

SUBMITTER: Wolfgang Wagner 

PROVIDER: E-GEOD-42807 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress