Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global Gene Expression Profiles of Cecropin B Induces Resistance in Haemophilus parasuis

ABSTRACT: To understand the mechanism of the adaptations, global gene expression profiles of the cecropin B--resistant strains of Haemophilus parasuis, The development of cecropin B (CB) resistance in H. parasuis SH0165 by exposing SH0165 using various concentrations of CB was investigated. One colony of bacterial cultures grown on TSA containing 30M-NM-0.3 (12 h). The H. parasuis CBR30 was normal culture with 30M-NM-0.3 (20 h). The H. parasuis CBR30-50 was normal culture with no cecropin B at OD600 >0.3 (12 h). Three independent experiments were performed on each group using a different sample for each experiment.

ORGANISM(S): Haemophilus parasuis

SUBMITTER: chunmei wang

PROVIDER: E-GEOD-42814 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Global Gene Expression Profiles of Cecropin B Induces Resistance in Haemophilus parasuis

Project description:To understand the mechanism of the adaptations, global gene expression profiles of the cecropin B--resistant strains of Haemophilus parasuis, The development of cecropin B (CB) resistance in H. parasuis SH0165 by exposing SH0165 using various concentrations of CB was investigated. One colony of bacterial cultures grown on TSA containing 30μg/mL CB was named CBR30, CBR30 cultured in TSA without CB for 50 passages was named CBR30-50. we used microarray technology to analyze the variation of the H. parasuis SH0165 transcriptional profile in CBR30 and CBR30-50. Our analysis to identify several genes whose system could be involved in inorganic ion transport, amino acid transport, and metabolism. Quantitative PCR was used to validate the differential expression of selected genes.

2012-12-11 | GSE42814 | GEO

Microarray analysis of Haemophilus parasuis SH0165 genomics induced by tilmicosin

Project description:To gain an extensive understanding of the molecular mechanisms of tilmicosin, we can begin by understanding the different regulated responses of bacteria to tilmicosin and studying the physiological activity of bacteria. In this study, we used microarray technology to analyze the variation of the H. parasuis SH0165 transcriptional profile in response to inhibitory and sub-inhibitory concentrations of tilmicosin. Our analysis to identify several genes whose system could be involved in the protection from antibiotic stress targeting the ribosome 50 subunit and help to confer the basal level of H. parasuis responses to antibacterial drugs, such as heat shock protein, ribosome and ribosome related transcription genes, the cell wall biogenesis genes. Quantitative RT-PCR was used to validate the differential expression of fifteen selected genes. H. parasuis SH0165 was induced by 0.25Âµg/ml tilmicosin at OD600 >0.3 (16 h). The 8Âµg/ml tilmicosin treatment was 50 mins at OD600 >0.3 (12 h). The H. parasuis SH0165 control group was normal culture with no tilmicosin at OD600 >0.3 (12 h). Three independent experiments were performed on each group using a different sample for each experiment.

2012-11-10 | E-GEOD-34744 | biostudies-arrayexpress

B. thetaiotaomicron before and after lactose addition to tryptone media 1

Project description:B. thetaiotaomicron was grown in a batch culture fermenter containing tryptone media. Lactose (final concentration 0.2%) was added at OD600 = 0.3. Samples were taken immediately before and 60 minutes after addition. Keywords: time course

2006-09-01 | GSE5276 | GEO

B. thetaiotaomicron before and after galactose addition to tryptone media

Project description:B. thetaiotaomicron was grown in a batch culture fermenter containing tryptone media. Galactose (final concentration 0.2%) was added at OD600 = 0.3. Samples were taken immediately before and 60 minutes after addition. Keywords: time course

2006-09-01 | GSE5275 | GEO

B. thetaiotaomicron before and after glucose addition to tryptone media

Project description:B. thetaiotaomicron was grown in a batch culture fermenter containing tryptone media. Glucose (final concentration 0.2%) was added at OD600 = 0.3. Samples were taken immediately before and 60 minutes after addition. Keywords: time course

2006-09-01 | GSE5274 | GEO

B. thetaiotaomicron before and after lactose addition to tryptone media 2

2006-09-01 | GSE5277 | GEO

T4 protein MotB expression in Escherichia coli, HPLC-MS

Project description:We investigated the effect of the T4 MotB protein on E. coli gene expression. E. coli BL21 (DE3) containing either pNW129 or pNW129-MotB were grown to early log phase (OD600 ~ 0.3) then induced with 0.2% arabinose for 20 minutes. T4 phage added to the culture at MOI10. Cells were then harvested at 5 min.

2022-10-13 | PXD036347 | Pride

Pseudomonas aeruginosa PA14 exposed to gliotoxin versus solvent control

Project description:Pseudomonas aeruginosa PA14 was precultured in SCFM2, and the OD600 was adjusted to 0.05 in 8 ml of SCFM2, in 6-well flat bottom cell culture plates. Gliotoxin (20 µM) or MeOH (solvent control) was added at log phase (optical density at 600 nm [OD600] = 0.3), and the cultures were incubated statically for an additional 4 h. 5 ml of each culture (triplicate) was then immediately added to an equal volume of RNA-later.

2023-03-30 | E-MTAB-12785 | biostudies-arrayexpress

Jacobsen2010

Project description:A pre-culture in NGY (overnight 30M-0C) was used to inoculate RPMI-1640 medium to an initial optical density at 600 nm (OD600) of 0.05. Cells were then grown to mid exponential phase (OD600 = 0.5M-^V0.6) at the appropriate temperature (25M-0C or 35M-0C) before the culture was<br><br>split into two aliquots and fluconazole (32 ?g mlM-^V1 final concentration) added to one; the other serving as the control. After 2 h the cells were harvested by centrifugation and flash<br><br>frozen in liquid nitrogen. Three independent cultures were grown for RNA extraction for each isolate at each condition. RNA was prepared from cell pellets with TRIZOLM-. reagent (Invitrogen) and a mechanical disruption method.

2011-11-16 | E-MEXP-3000 | biostudies-arrayexpress

Probing the regulatory effects of specific mutations in three major binding domains of the pleiotropic regulator CcpA of Bacillus subtilis

Project description:Study the effect of single amino acid point mutations in CcpA at the transcriptome level. Cells were grown in LB with or without 1% glucose and harvested at an OD600 of 0.3 (early exponential phase).

2015-12-24 | GSE69575 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data