Using RNA-Seq to Profile Soybean Seed Development from Fertilization to Maturity

ABSTRACT: To understand gene expression networks leading to functional properties and compositional traits of the soybean seed, we have undertaken a detailed examination of soybean seed development from a few days post-fertilization to the mature seed using Illumina high-throughput transcriptome sequencing (RNA-Seq). RNA was sequenced from seven different stages of seed development, yielding between 12 million and 78 million sequenced transcripts. These have been aligned to the 79,000 gene models predicted from the soybean genome recently sequenced by the Department of Energy Joint Genome Institute. Over one hundred gene models were identified with high expression exclusively in young seed stages, starting at just four days after fertilization. These were annotated as being related to many basic com

ORGANISM(S): Glycine max

SUBMITTER: Lila Vodkin 

PROVIDER: E-GEOD-42871 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress