Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Gene expression profiles of Tet1 knockout (T1KO), Tet2 knockout (T2KO) and Tet1/Tet2 double knockout (DKO) mouse embryonic stem cells.


ABSTRACT: Global gene expression profile of single and double mutant mouse ES cells were compared to wt ES cells. Two male Tet1 KO, one male Tet2 KO, two male double KO, two female double KO, two male WT and two female WT mouse ES cells were compared. Global gene expression profile of single and double knockout mouse embryonic stem cells were compared to that of wild type mouse ES cells. All used ES lines were derived from C57/BL/6 mixed background mice. RNA from feeder free mutant mouse ES cells was competetively hybridized against RNA from WT ES cells. Same sex lines were compared. Two independent ES line of each genotype were used, with the exception of Tet2 KO ES cells where only one male line was used.

ORGANISM(S): Mus musculus

SUBMITTER: Meelad Dawlaty 

PROVIDER: E-GEOD-42991 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Combined deficiency of Tet1 and Tet2 causes epigenetic abnormalities but is compatible with postnatal development.

Dawlaty Meelad M MM   Breiling Achim A   Le Thuc T   Raddatz Günter G   Barrasa M Inmaculada MI   Cheng Albert W AW   Gao Qing Q   Powell Benjamin E BE   Li Zhe Z   Xu Mingjiang M   Faull Kym F KF   Lyko Frank F   Jaenisch Rudolf R  

Developmental cell 20130124 3


Tet enzymes (Tet1/2/3) convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) in various embryonic and adult tissues. Mice mutant for either Tet1 or Tet2 are viable, raising the question of whether these enzymes have overlapping roles in development. Here we have generated Tet1 and Tet2 double-knockout (DKO) embryonic stem cells (ESCs) and mice. DKO ESCs remained pluripotent but were depleted of 5hmC and caused developmental defects in chimeric embryos. While a fraction of double-mutan  ...[more]

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