Inducible knockdown of Plasmodium gene expression using the glmS ribozyme

ABSTRACT: Conventional reverse genetic approaches for study of Plasmodium malaria parasite gene function are limited, or not applicable. Hence, new inducible systems are needed. Here we describe a method to control P. falciparum gene expression in which target genes bearing a glmS ribozyme in the 3M-bM-^@M-2 untranslated region (3M-bM-^@M-2-UTR) are efficiently knocked down in transgenic P. falciparum parasites in response to exogenous glucosamine (GlcN) inducer. Using reporter genes, we show that the glmS ribozyme cleaves reporter mRNA in vivo leading to reduction in mRNA expression following GlcN treatment. GlcN-induced ribozyme activation also led to efficient reduction of reporter protein, which could be rapidly reversed by removing the inducer. The glmS ribozyme was validated as a reverse-genet

ORGANISM(S): Plasmodium falciparum

SUBMITTER: Philip Shaw 

PROVIDER: E-GEOD-43125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress