Transcription profiling of human trabecular meshwork cultured cells, non-glaucomatous and POAG tissue

ABSTRACT: The goal of this study was to contrast genome-wide gene expression profiles of cultured human trabecular meshwork (HTM) cells, to that of control and primary open angle glaucoma (POAG) HTM tissues. Total RNA from cultured HTM cells and HTM tissue dissected from control and POAG anterior segments fixed in RNA later™ was linearly amplified with the OvationTM Biotin RNA Amplification and Labeling System and individually hybridized to Affymetrix Human Genome U133 Plus 2.0 high density microarrays. Data analysis was performed using the GeneSpring Software 7.0. Selected genes showing significant differential expression were validated by Quantitative real-time PCR in nonamplified RNA. Cultured HTM cells retained the expression of some genes characteristic of the HTM tissue, including chitinase

ORGANISM(S): Homo sapiens

SUBMITTER: Pedro Gonzalez 

PROVIDER: E-GEOD-4316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress