Glucose Metabolism impacts the spatio-temporal onset and magnitude of HSC induction in vivo

ABSTRACT: Many pathways regulating blood formation have been elucidated, yet how each coordinates with embryonic biophysiology to modulate the spatio-temporal production of hematopoietic stem cells (HSCs) is currently unresolved. Here, we report that glucose metabolism impacts the onset and magnitude of HSC induction in vivo. In zebrafish, transient elevations in physiological glucose levels elicited dose-dependent effects on HSC development, including enhanced runx1 expression and hematopoietic cluster formation in the Aorta-Gonad-Mesonephros (AGM) region; embryonic-to-adult transplantation studies confirmed glucose increased functional HSCs. Glucose uptake was required to mediate the enhancement in HSC development; likewise, metabolic inhibitors diminished nascent HSC production and reversed gluco

ORGANISM(S): Danio rerio

SUBMITTER: Trista North 

PROVIDER: E-GEOD-43186 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress