Comparison of the expression profiles of the 630E strain after 8h, 14h and 38h in TY

ABSTRACT: The virulence factors of Clostridium difficile have been studied for many years, but the main in vivo pathogenesis processes of this bacterium has to be investigated. Especially, data on the early mechanisms of C. difficile adaptation to the host is not yet available. The objective of our study was to improve the understanding of the pathogenesis of C. difficile by the analysis of the genome-wide temporal expression of C. difficile genes during the first hours of infection. Three groups of 4 axenic mice each were challenged by vegetative cells of the 630 C. difficile strain, and sacrified at 8, 14, and 38 hours post-infection. Pure prokaryotic RNA was obtained from caecal bacteria. Comparative hybridizations on strain 630 microarrays were done using cDNA issued from an 8-hours in vitro cul

ORGANISM(S): [Clostridium] difficile

SUBMITTER: Monot Marc 

PROVIDER: E-GEOD-43304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress