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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of GFP-positive cancer stem-like cells(CSLC) derived from MDA-MB453 cells, compared with GFP-negative non-cancer stem cells (non-CSLC).

ABSTRACT: Analysis of genes that differentially expressed in CSLC cells compared with non-CSLC cells, which are derived from the same parental MDA-MB453 cell line. Total RNAs were isolated from two pairs of GFP-positive CSLCs (ST1, ST2) and GFP-negative non-CSLCs (NS1, NS2) on different days. Two batches of RNAs were combined for microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Young Dong Yoo

PROVIDER: E-GEOD-43336 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular characteristics of cancer stem-like cells derived from human breast cancer cells.

Yoo Young Dong YD Han Dong Hoon DH Jang Jun Min JM Zakrzewska Adriana A Kim Seog-Young SY Choi Cheol Yong CY Lee Yong Jun YJ Kwon Yong Tae YT

Anticancer research 20130301 3

We characterized the cellular properties of cancer stem-like cells (CSLCs) isolated from immortalized MDA-MB453 human breast cancer cells in culture. We showed that although the expression of Octamer-binding transcription factor-4 (OCT4) correlates to stemness in these CSLCs, OCT4 knockdown does not induce their differentiation. Our results suggest that the differentiation program in MDA-MB453 CSLCs is blocked at a step upstream of the transcription of the OCT4 promoter, allowing CSLCs to mainta ...[more]

PMID: 23482743

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