DNaseI/Array GM06990
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Michael Kuehn
PROVIDER: E-GEOD-4334 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Genome-scale mapping of DNase I sensitivity in vivo using tiling DNA microarrays.
Sabo Peter J PJ Kuehn Michael S MS Thurman Robert R Johnson Brett E BE Johnson Ericka M EM Cao Hua H Yu Man M Rosenzweig Elizabeth E Goldy Jeff J Haydock Andrew A Weaver Molly M Shafer Anthony A Lee Kristin K Neri Fidencio F Humbert Richard R Singer Michael A MA Richmond Todd A TA Dorschner Michael O MO McArthur Michael M Hawrylycz Michael M Green Roland D RD Navas Patrick A PA Noble William S WS Stamatoyannopoulos John A JA
Nature methods 20060701 7
Localized accessibility of critical DNA sequences to the regulatory machinery is a key requirement for regulation of human genes. Here we describe a high-resolution, genome-scale approach for quantifying chromatin accessibility by measuring DNase I sensitivity as a continuous function of genome position using tiling DNA microarrays (DNase-array). We demonstrate this approach across 1% ( approximately 30 Mb) of the human genome, wherein we localized 2,690 classical DNase I hypersensitive sites wi ...[more]
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