DNA Methylation Biomarkers for Noninvasive Diagnosis of Colorectal Cancer

ABSTRACT: DNA methylation in colorectal cancer diagnosis. The Illumina GoldenGate Methylation Cancer Panel I was used to select a set of candidates markers informative of colorectal cancer diagnosis from 807 cancer-related genes. In the discovery phase, tumor tissue and paired adjacent normal mucosa from 92 colorectal patients were analyzed. Bisulphite converted DNA from 92 colorectal tumor samples and paired adjacent normal mucosa were hybridised to the Illumina GoldenGate Methylation Cancer Panel I. Additionally, replicates were hybridised for five tumor tissue and their corresponding normal mucosa for reproducibility purposes, totalling 194 samples. Three samples (SAMPLEs 49, 51, and 162) and 50 loci did not reach the quality criteria required regarding the signal-to-noise ratio and were therefor

ORGANISM(S): Homo sapiens

SUBMITTER: Antonio Berenguer-Llergo 

PROVIDER: E-GEOD-43369 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress