The Effect of the Leptin Receptor Q223R Polymorphism on the Host Transcriptome Following Infection with E. histolytica.

ABSTRACT: Resistance to amebiasis is associated with a polymorphism in the leptin receptor. Previous studies demonstrated that humans with the ancestral Q223 leptin receptor allele were nearly four times less likely to be infected with Entamoeba histolytica than those carrying the mutant R223 allele. We hypothesized that the Q223 allele protected against E. histolytica via STAT3-mediated transcription of genes required for mucosal immunity. To test this, mice containing the humanized LEPR Q or R allele at codon 223 were intracecally infected with E. histolytica. Susceptibility to amebiasis was assessed, and cecal tissues analyzed for changes in gene expression. By 72 h post-challenge all Q223 mice had cleared E. histolytica, whereas 39% of 223R mice were infected. 37 genes were differentially expres

ORGANISM(S): Mus musculus

SUBMITTER: Dan Sturdevant 

PROVIDER: E-GEOD-43372 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress