Molecular and functional features demarcate embryonic and induced pluripotent stem cells in rabbits

ABSTRACT: Little is known about the molecular and functional features of pluripotent stem cells (PSCs) in rabbits. To address this, we derived and characterized two types of rabbit PSCs from the same breed of New Zealand White rabbits: four lines of embryonic stem cells (rbESCs), and three lines of induced PSCs (rbiPSCs) that resulted from reprogramming adult skin fibroblasts. All lines were dependent on fibroblast growth factor 2. All rbESC lines exhibited molecular and functional properties typically associated with primed pluripotency. The rbESC lines also exhibited a cell cycle with a prolonged G1 phase and a DNA damage checkpoint prior to entry into the S phase, which are two features typically associated with the somatic cell cycle. In contrast, the rbiPSC lines exhibited some characteristics

ORGANISM(S): Oryctolagus cuniculus

SUBMITTER: barbara schmaltz-barbara 

PROVIDER: E-GEOD-43403 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress