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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition.

ABSTRACT: We reported the transcriptional profiles of E.coli expressing antimicrobial peptide LL37 under stress response condition. 4 samples, two groups, one group is under aerobic condition, the other group is under anaerobic condition. One of samples is E.coli which expressed LL37 as induction in each group, another sample is E.coli with no LL37 expression in vivo as control in each group.

ORGANISM(S): Escherichia coli

SUBMITTER: WEI LIU

PROVIDER: E-GEOD-43408 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effect of intracellular expression of antimicrobial peptide LL-37 on growth of escherichia coli strain TOP10 under aerobic and anaerobic conditions.

Liu Wei W Dong Shi Lei SL Xu Fei F Wang Xue Qin XQ Withers T Ryan TR Yu Hongwei D HD Wang Xin X

Antimicrobial agents and chemotherapy 20130715 10

Antimicrobial peptides (AMPs) can cause lysis of target bacteria by directly inserting themselves into the lipid bilayer. This killing mechanism confounds the identification of the intracellular targets of AMPs. To circumvent this, we used a shuttle vector containing the inducible expression of a human cathelicidin-related AMP, LL-37, to examine its effect on Escherichia coli TOP10 under aerobic and anaerobic growth conditions. Induction of LL-37 caused growth inhibition and alteration in cell m ...[more]

PMID: 23856776

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Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition.

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