Reconstructing de novo silencing of an active plant retrotransposon: dynamics, mechanisms, biological implications

ABSTRACT: In plants, maintenance-methylation mediated by METHYLTRANSFERASE-1 (MET1), siRNA-directed de-novo methylation, and chromatin remodeling by DECREASE IN DNA METHYLATION -1 (DDM1) promote transcriptional gene-silencing of transposable elements (TEs). This process is mostly investigated at steady states reflecting how long-established silent conditions are maintained, faithfully re-iterated or temporarily modified during growth, stress and over generations. How invasive TEs are detected and silenced de novo, however, remains largely unknown. Using inbred lineages of hybrid Arabidopsis epigenomes combining wild-type and met1 or ddm1 chromosomes, we have deciphered the timing, spatial distribution and mechanisms underpinning the proliferation and eventual demise of the endogenous retrotransposon

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Antonin Marchais 

PROVIDER: E-GEOD-43412 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress