Base-pair level resolution of the Nasonia vitripennis methylome

ABSTRACT: The parasitoid wasp Nasonia vitripennis is an emerging genetic model for functional analysis of DNA methylation. Here, we characterize genome-wide methylation at a base-pair resolution, and compare these results to gene expression across five developmental stages and to methylation patterns reported in other insects. An accurate assessment of DNA methylation across the genome is accomplished using bisulfite sequencing of adult females from a highly inbred line. One-third of genes show extensive methylation over the gene body, yet methylated DNA is not found in non-coding regions and rarely in transposons. Methylated genes occur in small clusters across the genome. Methylation demarcates exon-intron boundaries, with elevated levels over exons, primarily in the 5’ regions of genes. It is als

ORGANISM(S): Nasonia vitripennis

SUBMITTER: Andrew Clark 

PROVIDER: E-GEOD-43423 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress