Extensive changes in DNA methylation are associated with expression of mutant huntingtin [MeDIP-seq]

ABSTRACT: The earliest stages of Huntington’s disease are marked by changes in gene expression that are caused in an indirect and poorly understood manner by polyglutamine expansions in the huntingtin protein (HTT). To explore the hypothesis DNA methylation may be altered in cells expressing mutated HTT, we use reduced-representation bisulfite sequencing (RRBS) to map sites of DNA methylation in cells carrying either wild-type or mutant HTT. We find that a large fraction of the genes that change in expression in the presence of mutant huntingtin demonstrate significant changes in DNA methylation. Regions with low CpG content, which have previously been shown to undergo methylation changes in response to neuronal activity, are disproportionately affected. Based on the sequence of regions that cha

ORGANISM(S): Mus musculus

SUBMITTER: Christopher Ng 

PROVIDER: E-GEOD-43430 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress