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Expression data from laser captured gastric neck cells and zymogenic cell from wild type and MIST1 knockout mice

ABSTRACT: MIST1 is a bHLH transcription factor that is necessary for the maturation of gastric zymogenic cells as they differentiate from their precursor mucous neck cells. In this experiment, mucous neck cells and zymogenic cells of normal, adult C57BL/6 and MIST1 knockout mice were laser-capture microdissected in order to determine MIST1-dependent, zymogenic cell specific gene expression. Stomachs were excised immediately following sacrifice, quickly flushed with room-temperature PBS, inflated by duodenal injection of OCT compound, frozen in Cytocool II, and cut into serial 7-M-NM-

ORGANISM(S): Mus musculus

SUBMITTER: Jason Mills

PROVIDER: E-GEOD-43441 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from laser captured gastric neck cells and zymogenic cell from wild type and MIST1 knockout mice

Project description:MIST1 is a bHLH transcription factor that is necessary for the maturation of gastric zymogenic cells as they differentiate from their precursor mucous neck cells. In this experiment, mucous neck cells and zymogenic cells of normal, adult C57BL/6 and MIST1 knockout mice were laser-capture microdissected in order to determine MIST1-dependent, zymogenic cell specific gene expression.

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