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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from laser captured gastric neck cells and zymogenic cell from wild type and MIST1 knockout mice

ABSTRACT: MIST1 is a bHLH transcription factor that is necessary for the maturation of gastric zymogenic cells as they differentiate from their precursor mucous neck cells. In this experiment, mucous neck cells and zymogenic cells of normal, adult C57BL/6 and MIST1 knockout mice were laser-capture microdissected in order to determine MIST1-dependent, zymogenic cell specific gene expression. Stomachs were excised immediately following sacrifice, quickly flushed with room-temperature PBS, inflated by duodenal injection of OCT compound, frozen in Cytocool II, and cut into serial 7-M-NM-

ORGANISM(S): Mus musculus

SUBMITTER: Jason Mills

PROVIDER: E-GEOD-43441 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression data from laser captured gastric neck cells and zymogenic cell from wild type and MIST1 knockout mice

Project description:MIST1 is a bHLH transcription factor that is necessary for the maturation of gastric zymogenic cells as they differentiate from their precursor mucous neck cells. In this experiment, mucous neck cells and zymogenic cells of normal, adult C57BL/6 and MIST1 knockout mice were laser-capture microdissected in order to determine MIST1-dependent, zymogenic cell specific gene expression.

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2008-06-13 | E-GEOD-5018 | biostudies-arrayexpress

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Project description:The transcription factor MIST1 is required for final maturation of secretory cells of diverse tissues, including gastric digestive-enzyme secreting zymogenic (chief) cells (ZCs). Here, we show that MIST1 directly activates RAB26, RAB3D and several other genes. Experiment Overall Design: We used microarrays to determine genes upregulated following transient MIST1 transfection. Two gastric cell lines were used: HGC-27 and AGS. Three chips were generated from each cell line: parental (untransfected), GFP+empty vector (a control for effects of transfection), and GFP+MIST1. The latter two chips were generated from cells flow sorted based on GFP fluorescence to isolate cells with high levels of transfection. Chips were analyzed by dChip to identify genes expressed in both MIST1-transfected cell populations relative to their respective controls in multiple Boolean 'AND' comparisons.

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Forced Expression of MIST1 in Hepatocytes

Project description:MIST1 over-expression has not been analyzed in a cell type that does not express endogenous MIST1. Here, we force-express ectopic MIST1 in the hepatocytes of mouse livers. We extracted mRNA and compared gene expression levels between MIST1-expressing mouse livers to controls.

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Forced Expression of MIST1 in Parietal Cells

Project description:MIST1 over-expression has not been analyzed in a cell type that does not express endogenous MIST1. Here, we force-express ectopic MIST1 in the parietal cells of mouse stomachs. We extracted whole corpus stomach mRNA and compared gene expression levels between MIST1-expressing mouse stomachs to controls.

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