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The effects of M3 muscarinic receptor gene ablation on murine colon neoplasia and gene expression

ABSTRACT: In mice treated with azoxymethane (AOM), an intestine-selective carcinogen, both colon tumor number and size were robustly reduced in Chrm3-/- compared to WT mice. In the present study, a microarray approach was used to identify genes that might have mechanistic relevance regarding the effects of M3R expression and activation on colon neoplasia, and also to identify potential biomarkers and therapeutic targets for colon cancer Total RNA obtained from isolated colon tumor in WT mice compared to M3KO mice.

ORGANISM(S): Mus musculus

SUBMITTER: Kunrong Cheng

PROVIDER: E-GEOD-43444 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The effects of M3 muscarinic receptor gene ablation on murine colon neoplasia and gene expression

Project description:In mice treated with azoxymethane (AOM), an intestine-selective carcinogen, both colon tumor number and size were robustly reduced in Chrm3-/- compared to WT mice. In the present study, a microarray approach was used to identify genes that might have mechanistic relevance regarding the effects of M3R expression and activation on colon neoplasia, and also to identify potential biomarkers and therapeutic targets for colon cancer

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Project description:To find out which mRNAs are significantly differential expression and potentially involved in the process of inflammation promoting carcinogenesis of colorectal cancer (CRC). We established a colitis-associated CRC (AOM/DSS, Azoxymethane/Dextran sulfate sodium salt) model, colitis (DSS) model and high dose carcinogen (AOM, about 5 times AOM amount given than AOM/DSS model) model. At day 100 when tumor formed in AOM/DSS bearing mice (colitis-associated CRC mice) but no tumor was found in AOM (high dose carcinogen) and DSS model, we employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential to involve in the progression of CRC promoted by inflammation. 5-7 weeks female BALB/c mice, (1) AOM/DSS group: AOM 12.5mg/kg i.p. at day 1, DSS drinking 5d/21dx3circles from day 5; (2) AOM group: AOM 10mg/kg i.p. 1/weekx6 from day 1; (3) DSS group: DSS drinking 5d/21dx3circles from day 5. The distal colon epithelial tissues were collected at day100 when tumor formed in AOM/DSS bearing mice. The whole genome microarray expression profiling experiments were performed together.

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