Estrogen Induced DNA Methylation Changes in Mouse Hippocampus

ABSTRACT: Estrogen, specifically estradiol, has been shown to mediate DNA methylation changes at mutiple locations downstream of estrogen response elements (EREs). We identified differentially methylated regions associated with long-term estradiol exposure in the hippocampus of C57BL/6J mice. Hippocampus was collected from ovariectomized (OVX) C57BL/6J mice treated with estradiol (n=5) and vehicle controls (n=5). Genomic DNA was extracted and treated with methylation sensitive restriction enzymes HpaII and HinP1I to enrich for the unmethylated fraction. This fraction was interrogated using the Affymetrix GeneChip® Mouse Tiling Promoter 1.0R Array

ORGANISM(S): Mus musculus

SUBMITTER: Jerry Guintivano 

PROVIDER: E-GEOD-43460 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress