Role of HGF in epithelial-stromal cell interactions during progression from benign breast disease to ductal carcinoma in situ

ABSTRACT: ABSTRACT: Introduction: Basal-like and luminal breast cancers have distinct stromal-epithelial interactions, which likely play a role in progression to invasive cancer. However, little is known about how stromal-epithelial interactions evolve in benign and pre-invasive lesions.Methods: To study epithelial-stroma interactions in basal-like breast cancer progression, we cocultured reduction mammoplasty fibroblasts with the isogenic MCF10 series of cell lines (representing benign/normal, atypical hyperplasia, and ductal carcinoma in situ). We used gene expression microarrays to identify pathways induced by coculture in premalignant cells (MCF10DCIS) compared to normal and benign (MCF10A and MCF10AT1). Relevant pathways were then (1) targeted in vitro and effects on morphogenesis were evaluate

ORGANISM(S): Homo sapiens

SUBMITTER: Melissa Troester 

PROVIDER: E-GEOD-43467 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress