Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Differentially expressed genes in H2AX knockdown cells undergoing apoptosis

ABSTRACT: H2AX has been characterized as a novel tumor suppressor protein. Difficiency of H2AX will result in apoptotic inhibition of cancer cells. However, how H2AX epigenetically regulates apoptosis of cancer cells is still unclear. To reveal the genes expression regulated by H2AX and involved in apoptosis, the microarray profiling analysis was employed to identify differentially expressed genes in H2AX knockdown lung cancer cells and control ones after apoptotic induction. H2AX was knockdown by miRNA interfering system in human lung cancer originated cell A549 and the stable cell line named P1, while the control stable cell line named C. Genes with greater than 1.2-fold change and P-value ＜0.05 were identified as differentially expressed genes between C and P1 cells both with apoptosis induction. The two groups including control (C) and H2AX knockdown lung cancer cells (P1) were harvested 48 h after VP-16 treatment. Three independent experiments were performed for each group.

ORGANISM(S): Homo sapiens

SUBMITTER: Chengrong Lu 

PROVIDER: E-GEOD-43498 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Genome-wide transcriptional analysis of apoptosis-related genes and pathways regulated by H2AX in lung cancer A549 cells.

Lu Chengrong C   Xiong Min M   Luo Yuan Y   Li Jing J   Zhang Yanjun Y   Dong Yaqiong Y   Zhu Yanjun Y   Niu Tianhui T   Wang Zhe Z   Duan Lianning L  

Apoptosis : an international journal on programmed cell death 20130901 9

Histone H2AX is a novel tumor suppressor protein and plays an important role in apoptosis of cancer cells. However, the role of H2AX in lung cancer cells is unclear. The detailed mechanism and epigenetic regulation by H2AX remain elusive in cancer cells. We showed that H2AX was involved in apoptosis of lung cancer A549 cells as in other tumor cells. Knockdown of H2AX strongly suppressed apoptosis of A549 cells. We clarified the molecular mechanisms of apoptosis regulated by H2AX based on genome-  ...[more]

Similar Datasets

Transcriptomics Differentially expressed miRNAs in H2AX knockdown cells undergoing apoptosis
2015-04-25 | E-GEOD-68233 | biostudies-arrayexpress
Unknown Differentially expressed genes in H2AX knockdown cells undergoing apoptosis
2013-01-16 | GSE43498 | GEO
Unknown Differentially expressed miRNAs in H2AX knockdown cells undergoing apoptosis
2015-04-25 | GSE68233 | GEO
Genomics Homo sapiens
| PRJNA282083 | ENA
Genomics,Multiomics Homo sapiens
| PRJNA186599 | ENA
Genomics The P1 Transcription Factor Binding to the Genome
2012-09-14 | E-GEOD-38587 | biostudies-arrayexpress
Genomics,Multiomics E-GEOD-43498
2015-08-19 | E-GEOD-43498 | ExpressionAtlas
Proteomics The Whi2p-Psr1p/Psr2p complex regulates interference competition and expansion of cells with competitive advantage in multicellular yeast colonies - Proteomics of P1, P1-whi2 and P1-psr1psr2 colonies.
2020-06-04 | PXD016670 | Pride
Transcriptomics Gene expression profiling from cohesin knockdown cells obtained grown in methylcellulose
2015-09-16 | E-GEOD-72368 | biostudies-arrayexpress
Transcriptomics Differentially expressed genes in metallopanstimulin-1 knockdown cells
2011-04-27 | E-GEOD-28877 | biostudies-arrayexpress