Transcription profiling of muscles from mice subjected to a single bout of eccentric contractions to understand the molecular mechanism of eccentric contraction-induced injury.

ABSTRACT: Mice were subjected to 50 eccentric contractions (EC) or 50 isometric contractions (IC) using a non-invasive model, and then sacrificed 48 hours later. RNA from the tibialis anterior of 4 animals were pooled and then split into two groups for hybridization onto two separate Affymetrix MGU74Av2 chips. Control samples were contralateral to the exercised legs, and were only subjected to enough contractions to measure isometric torque. Eccentric contractions (ECs), in which a muscle is forced to lengthen while activated, result in muscle injury and, eventually, muscle strengthening and prevention of further injury. Although the mechanical basis of eccentric contraction-induced injury has been studied in detail, muscle's biological response is less well characterized. This study presents the de

ORGANISM(S): Mus musculus

SUBMITTER: Richard Louis Lieber 

PROVIDER: E-GEOD-435 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress