Chloroplast-dependent programmed cell death is activated in Arabidopsis cell cultures after singlet oxygen production by Rose Bengal

ABSTRACT: Arabidopsis thaliana cell suspension cultures (ACSC) were subjected to 30-min, mild chemical treatments with three different singlet oxygen elicitors at low-medium light conditions (150 µE m–2 s–1) with the aim of getting a better understanding of singlet oxygen-mediated defence responses in plants. The three elicitors Indigo Carmine (IC), Methylene Violet (MV) and Rose Bengal (RB) at a concentration of 0.5 µM were chosen because they exhibited different abilities to permeate the plasma membrane and to accumulate in the cell soma or organelles such as chloroplasts. In addition, ACSC were treated with 500 µM H2O2 for comparison. Confocal image analysis of Arabidopsis cells revealed that IC was not retained in cells, whereas MV and RB permeated the plasma membrane and accumulated in the chlo

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: FRANCISCO GARCIA 

PROVIDER: E-GEOD-43551 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress