Genome-wide localization of Smc1, Smc3 and CTCF in mouse B cells.

ABSTRACT: Immunoglobulin class switch recombination (CSR) is initiated by the transcription-coupled recruitment of activation induced cytidine deaminase (AID) to switch regions and by the subsequent generation and resolution of dsDNA breaks (DSBs). During, CSR the IgH locus undergoes dynamic three-dimensional structural changes in which promoters, enhancers and switch regions are brought to close proximity. Nevertheless, little is known about the underlying mechanisms. Here we show that during CSR, AID associates with subunits of cohesin, a complex previously implicated in DNA repair and in the formation of DNA loops between enhancers and promoters. By ChIP-Seq experiments, we find that Cohesin is dynamically recruited to the IgH locus during CSR and that knockdown of Cohesin or its regulatory subun

ORGANISM(S): Mus musculus

SUBMITTER: Anne-Sophie THOMAS 

PROVIDER: E-GEOD-43594 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress