Dual Roles of the Transcription Factor Grainyhead-like 2 (GRHL2) in Breast Cancer

ABSTRACT: Using a retrovirus-mediated cDNA expression cloning approach we identified the grainyhead-like 2 (GRHL2) transcription factor as novel protooncogene. Overexpression of GRHL2 in NIH3T3 cells induced striking morphological changes, an increase in cell proliferation, anchorage-independent growth, and tumor growth in vivo. By combining a microarray analysis and a phylogenetic footprinting analysis with various biochemical assays we identified the epidermal growth factor receptor family member Erbb3 as a novel GRHL2 target gene. In breast cancer cell lines, shRNA-mediated knockdown of GRHL2 expression or functional inactivation of GRHL2 using dominant-negative GRHL2 proteins induce downregulation of ERBB3 gene expression, a striking reduction in cell proliferation and morphological and phenotyp

ORGANISM(S): Mus musculus

SUBMITTER: Christian Schulze 

PROVIDER: E-GEOD-43610 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress