A Genome-Wide Survey of Maternal and Embryonic Transcripts during Xenopus tropicalis Development

ABSTRACT: To analyze the dynamics and diversity of coding and non-coding transcripts during development, both polyadenylated mRNA and ribosomal RNA-depleted total RNA were harvested across six developmental stages and subjected to high throughput sequencing. The maternally loaded transcriptome is highly diverse and consists of both polyadenylated and deadenylated transcripts. Many maternal genes show peak expression in the oocyte and include genes which are known to be the key regulators of events like oocyte maturation and fertilization. Of all the transcripts that increase in abundance between an early blastula and larval stages, about 30% of the embryonic genes are induced by fourfold or more by the late blastula stage and another 35% by late gastrulation. Using a gene model validation and discov

ORGANISM(S): Xenopus (Silurana) tropicalis

SUBMITTER: Gert Jan Veenstra 

PROVIDER: E-GEOD-43652 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress