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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Dynamics of TRIM33 binding in macrophages and effects of TRIM33 deletion on chromatin state during activation of primary macrophages.

ABSTRACT: To get insight into TRIM33 functions, TRIM33 ChIP-seq was carried out in murine macrophage cell line (RAW) and in bone marrow-derived macrophages (BMDM). The results showed that, in addition to its role in hematopoietic differentiation, TRIM33 may modulate PU.1 transcriptional activity during macrophage development and/or activation.To characterize the role of TRIM33 in macrophages, we bred TRIM33fl/fl mice with Lyz-Cre mice where the Cre recombinase gene is under the regulatory sequences of the Lyz gene that is expressed only in mature myeloid cells. Bone marrow cells from LyzCre/Trim33+/+ mice and LyzCre/Trim33flox/flox mice were differentiated in macrophages and treated during 0h, 4h, 12h and 24h with LPS. Using ChIP-seq, we provide a link between TRIM33 binding and H3K4me3 spreading on inflammatory genes in macrophages. Chromatin immunoprecipitations of TRIM33 and H3K4Me3 followed by multiparallel sequencing performed in murine bone marrow-derived macrophages (BMDM).

ORGANISM(S): Mus musculus

SUBMITTER: federica ferri

PROVIDER: E-GEOD-43654 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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TRIM33 binding in hematopoietic cell lines and effects of TRIM33 deletion on RNA PolII occupancy during activation of primary macrophages

Project description:To get insight into lineage-specific TRIM33 functions, TRIM33 ChIP-seq was carried out in non-differentiated erythroid (MEL NI), differentiated erythroid (MEL I), immature myeloid (32D). In addition, we compared the genome-wide profiles of RNA PolII occupancy between wild-type bone marrow-derived macrophages (BMDM) and Trim33-/- BMDM activated with LPS.

2021-09-17 | GSE73448 | GEO

Dynamics of TRIM33 binding in macrophages and effects of TRIM33 deletion on chromatin state during activation of primary macrophages.

Project description:To get insight into TRIM33 functions, TRIM33 ChIP-seq was carried out in murine macrophage cell line (RAW) and in bone marrow-derived macrophages (BMDM). The results showed that, in addition to its role in hematopoietic differentiation, TRIM33 may modulate PU.1 transcriptional activity during macrophage development and/or activation.To characterize the role of TRIM33 in macrophages, we bred TRIM33fl/fl mice with Lyz-Cre mice where the Cre recombinase gene is under the regulatory sequences of the Lyz gene that is expressed only in mature myeloid cells. Bone marrow cells from LyzCre/Trim33+/+ mice and LyzCre/Trim33flox/flox mice were differentiated in macrophages and treated during 0h, 4h, 12h and 24h with LPS. Using ChIP-seq, we provide a link between TRIM33 binding and H3K4me3 spreading on inflammatory genes in macrophages.

2015-11-23 | GSE43654 | GEO

H3K4me3 chromatin profiling in SUMOylation-competent and -deficient bone marrow derived dendritic cells unstimulated or stimulated with LPS

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Genome-wide analysis of RARÎ² transcriptional targets in mouse striatum links retinoic acid signaling with Huntingtonâs disease and other neurodegenerative disorders

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TRIM33 switches off Ifnb1 transcription during the late phase of macrophage activation

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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