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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene Expression of F. tularensis LVS in hepatocytic cell line FL83B vs LVS cultured in MH broth

ABSTRACT: Transcriptional profiling of LVS grown in hepatocytic cell line FL83B was compared to that of LVS cultued in MH broth to discover genes important for intracellular growth. Two-condition experiment, Intracellular vs. Broth. Biological replicates: 4 Broth, 4 intracellular, independently grown and harvested. One replicate per array.

ORGANISM(S): Francisella tularensis subsp. holarctica LVS

SUBMITTER: Cindy Thomas

PROVIDER: E-GEOD-43731 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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FeoB-mediated uptake of iron by Francisella tularensis.

Thomas-Charles Cindy A CA Zheng Huaixin H Palmer Lance E LE Mena Patricio P Thanassi David G DG Furie Martha B MB

Infection and immunity 20130528 8

Francisella tularensis, the bacterial cause of tularemia, infects the liver and replicates in hepatocytes in vivo and in vitro. However, the factors that govern adaptation of F. tularensis to the intrahepatocytic niche have not been identified. Using cDNA microarrays, we determined the transcriptional profile of the live vaccine strain (LVS) of F. tularensis grown in the FL83B murine hepatocytic cell line compared to that of F. tularensis cultured in broth. The fslC gene of the fsl operon was th ...[more]

PMID: 23716605

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Gene Expression of F. tularensis LVS in hepatocytic cell line FL83B vs LVS cultured in MH broth

Project description:Transcriptional profiling of LVS grown in hepatocytic cell line FL83B was compared to that of LVS cultued in MH broth to discover genes important for intracellular growth.

2013-01-25 | GSE43731 | GEO

Expression data from mouse liver infected with Ft LVS (without or with LPS pretreatment)

Project description:Background: It has been shown previously that administration of Francisella tularensis (Ft) LVS lipopolysaccharide (LPS) protects mice against subsequent challenge with Ft LVS and blunts the pro-inflammatory cytokine response. Methods: To investigate further the molecular mechanisms that underlie Ft LVS LPS-mediated protection, we profiled global hepatic gene expression following Ft LVS LPS or saline pretreatment and subsequent Ft LVS challenge using Affymetrix arrays. Results: A large number of genes (> 3000) were differentially expressed at 48 hours post-infection. The degree of modulation of inflammatory genes by infection was clearly attenuated by LPS-pretreatment in the surviving mice. However, LPS alone had a subtle but significant effect on the gene expression profile of the uninfected mice. By employing gene set enrichment analysis, we discovered significant up-regulation of peroxisome proliferator activated receptors (PPARs) and their target genes in LPS-treated liver. Conclusions: We hypothesize that the LPS-induced blunting of pro-inflammatory response in mouse is, in part, mediated by PPARs (alpha and gamma). Experiment Overall Design: Two groups of 9 mice each were used for this experiment. 48 hours prior to Ft LVS challenge, mice were injected i.p. with either 100 ng of Ft LVS LPS or an equivalent volume of saline. On the day of challenge, 3 saline- and 3 Ft LVS LPS-pretreated animals were sacrificed (uninfected controls), while all remaining mice were challenged i.p. with ~4-5 X 105 Ft LVS. Ft LVS-challenged mice were sacrificed (in groups of 3) at 24 and 48 hours post-infection.

2010-02-21 | E-GEOD-16207 | biostudies-arrayexpress

Novel Small RNA Modulates Virulence in Francisella tularensis

Project description:Most sRNAs control the expression of target genes by interacting with their mRNA. The fvr1 (Francisella virulence RNA 1) gene is found in the IGR between FTL_0777 and FTL_0778 and its sequence does not overlap those of the flanking genes, indicating that its target(s) is located in trans. Base-pairing between a sRNA and target mRNA generally leads to changed translation and often the stability of the mRNA is affected as well. Therefore, to identify possible targets of Fvr1, we compared the transcriptomes of the LVS?fvr1(LVS, for Live Vaccine Strain) and LVS/pfvr1+ strains after growth in liquid broth.

2012-01-31 | E-MEXP-3424 | biostudies-arrayexpress

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LVS iclR deletion mutant vs. wild-type LVS

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2010-08-13 | E-GEOD-23454 | biostudies-arrayexpress

Transcription profiling of wild type and relA spoT mutant Francisella tularensis

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