Intestinal label-retaining cells are secretory precursors expressing Lgr5

ABSTRACT: The epithelial lining of the small intestine is continuously renewed from a small number of stem cells including Lgr5 expressing crypt base cells that have been shown to be a rapidly cycling stem cell population in homeostasis. Alternative markers of intestinal stem cells have variously identified populations as rapidly cycling or quiescent with proposed roles for the latter as a parallel or reserve stem cell population. However, the exact nature of quiescent crypt cells remains unknown. Here by applying novel mouse models that permit their isolation and characterisation as label-retaining cells (LRCs), and for the first time performing lineage tracing from them, we show quiescent cells to be committed secretory precursors that are capable of recall to the stem cell state. We reveal LRCs t

ORGANISM(S): Mus musculus

SUBMITTER: Roslin Russell 

PROVIDER: E-GEOD-43772 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress