Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse hypogonadal testis that had been pretreated with testosterone proprionate for 2 weeks then submitted to testosterone withdrawal

ABSTRACT: Analysis of the transcriptome of testes of hypogonadal mice that had been pretreated with testosterone proprionate for 2 weeks then submitted to testosterone withdrawal.

ORGANISM(S): Mus musculus

SUBMITTER: Patricia Imele Sadate-Ngatchou

PROVIDER: E-GEOD-438 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of testosterone-regulated genes in testes of hypogonadal mice using oligonucleotide microarray.

Sadate-Ngatchou Patricia I PI Pouchnik Derek J DJ Griswold Michael D MD

Molecular endocrinology (Baltimore, Md.) 20031106 2

FSH and testosterone (T) are required for normal spermatogenesis in mammalian males. These hormones regulate the function of Sertoli cells, which in turn support the differentiation of germ cells in the seminiferous tubules. The molecular targets for these hormones in the testis remain elusive. In this study, we have used hypogonadal (hpg) mice as an in vivo model to examine the actions of T on gene expression in murine testis. This expression pattern was analyzed using Affymetrix Murine GeneChi ...[more]

PMID: 14605096

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Project description:Androgenic steroids are increasingly used for hormone therapy of postmenopausal women and abused as life style drugs and for doping purposes, though knowledge about associated health risks in females is very limited. In order to understand more about short- and long-term androgen effects on a molecular level, we have analyzed hepatic gene expression in female C57BL/6 mice immediately after subcutaneous treatment with testosterone for 3 weeks and after 12 weeks hormone withdrawal using Affymetrix array technology and quantitative real-time RT-PCR. Among about 14,000 genes examined, 48 were up- and 65 genes were downregulated by testosterone after 3-weeks treatment and about 50% of these changes persisted even 12 weeks after testostrone withdrawal. In addition to obvious risks such as induction of hepatocellular carcinomas and virilization of liver metabolism, testosterone induced a series of changes, as e.g. dysregulation of hepatic gene expression due to incomplete conversion of female to male phenotype â in particular downregulation of cytochrom P450 isoforms and sulfotransferases. As a long-term testosterone effect, transcripts emerged in the liver that are normally specific for the exocine pancreas including amylase 2, ribonuclease 1, and several trypsin-, chymotrypsin-, and elastase-like proteases. This transdifferentiation of hepatic to exocrine pancreatic tissue indicates that testosterone can initiate long-lasting differentiation programs, which â once induced â progress even after androgen withdrawal. This may have far-reaching consequences difficult to foresee implying long-term hazards of testosterone-treatment for female health that have not been taken into account yet. Mice were treated with testosterone or sesame oil (vehicle) for three weeks twice a week. Gene expression in the liver was analyzed either directly after treatment or after three weeks of hormone/vehicle withdrawal. For each of these four groups, three individual mice were used as biological replicates.

Dataset Information

Transcription profiling of mouse hypogonadal testis that had been pretreated with testosterone proprionate for 2 weeks then submitted to testosterone withdrawal

Publications

Identification of testosterone-regulated genes in testes of hypogonadal mice using oligonucleotide microarray.

Similar Datasets

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