Altered Expression of Intraflagellar Transport Genes is Associated with Short Cilia in Smoking and COPD

ABSTRACT: Study Smoking and COPD are associated with decreased mucociliary clearance and healthy smokers have shorter cilia in the large airway than nonsmokers. Intraflagellar transport (IFT) is the process by which cilia are produced and maintained. We assessed expression of IFT-related genes in smokers and nonsmokers and evaluated cilia length in the large and small airway of nonsmokers, healthy smokers, and smokers with COPD. Methods Airway epithelium was obtained via bronchoscopic brushing. Affymetrix microarrays were used to evaluate IFT gene expression in 2 independent data sets from large and small airway. Cilia length was assessed by measuring 100 cilia (10 cilia on each of 10 cells) per subject. Results All 40 IFT genes were expressed in the human large and small airway epithelium. In the l

ORGANISM(S): Homo sapiens

SUBMITTER: Yael Strulovici-Barel 

PROVIDER: E-GEOD-43939 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress