Reconstruction of the dynamic regulatory network that controls Th17 cell differentiation by systematic perturbation in primary cells (RNA-Seq)

ABSTRACT: Despite their enormous importance, the molecular circuits that control the differentiation of Th17 cells remain largely unknown. Recent studies have reconstructed regulatory networks in mammalian cells, but have focused on short-term responses and relied on perturbation approaches that cannot be applied to primary T cells. Here, we develop a systematic strategy – combining transcriptional profiling at high temporal resolution, novel computational algorithms, and innovative nanowire-based tools for performing gene perturbations in primary T cells – to derive and experimentally validate a temporal model of the dynamic regulatory network that controls Th17 differentiation. The network is arranged into two self-reinforcing and mutually antagonistic modules that either suppress or promote Th17

ORGANISM(S): Mus musculus

SUBMITTER: Nir Yosef 

PROVIDER: E-GEOD-43948 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress