Over-expression of CTR1 delta-300 alters element and transcription profiles in yeast

ABSTRACT: In an approach to generate Saccharomyces cerevisiae strains with increased intracellular copper amounts for technical applications we over-expressed the copper transporter CTR1 and a variant of CTR1 with a truncation in the C-terminus after the 300 amino acids (CTR1 delta-300). We determined the copper sensitivity of the generated strains and used inductively coupled plasma spectrometry (ICP-OES and ICP-MS) analysis to investigate the effects of over-expression of both constructs under excess copper on the cellular content of different elements in S. cerevisiae. In addition, we ran DNA microarray analysis to obtain the gene expression profile under the changed element contents. Over-expression of CTR1 increased the copper content in the cells up to 160% and 78 genes were differentially reg

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Astrid Schuller 

PROVIDER: E-GEOD-44043 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress