High resolution methylome analysis reveals widespread functional hypomethylation during adult human erythropoiesis

ABSTRACT: Differentiation of hematopoietic stem cells to red cells requires coordinated expression of numerous erythroid genes. To understand the regulatory mechanisms governing lineage commitment we conducted a high resolution methylomic and transcriptomic analysis of six major stages of human erythroid differentiation. We observed widespread epigenetic differences between early and late stages of erythropoiesis with progressive loss of methylation being the dominant change during differentiation. Gene bodies, intergenic regions and CpG shores were preferentially demethylated during erythropoiesis. Epigenetic changes at transcription factor binding sites correlated significantly with changes in gene expression and were enriched for binding motifs for SCL, MYB, GATA and other factors not previously

ORGANISM(S): Homo sapiens

SUBMITTER: Yiting Yu 

PROVIDER: E-GEOD-44054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress