Profiling of microRNA expression in hepatocellular carcinoma cells with overexpression of G protein alpha 12 active mutant

ABSTRACT: Heterotrimeric guanine nucleotide-binding proteins (G proteins) transmit extracellular signals from cell surface G protein-coupled receptors to intracellular effector molecules. Attention has been paid to the GM-NM-112 family members because they mediate cell invasion, migration, and tumorigenesis. In our findings, GM-NM-112 levels were higher in human HCCs than non-tumorous liver tissues. Array analyses using Huh7 cells stably transfected with GM-NM-112QL (an active mutant form) enabled us to extract the microRNAs dysregulated by GM-NM-112 active mutant. Of them, miR-122 was most greatly decreased. In addition, we found decreases of miR-200b/a, miR-192/215 levels. Dysregulation of the microRNAs changed the levels of key proteins associated with HCC cell migration/invasion. Huh7 hepatoma c

ORGANISM(S): Homo sapiens

SUBMITTER: Sang Geon Kim 

PROVIDER: E-GEOD-44079 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress